This webinar will cover:

• What is KBase?
• Platform setup
• How to use data and apps in the Narrative

Learning Goals

• Set up their KBase accounts
• Join the workshop Org
• Practice using KBase

Materials
Slides
Introductory Activity
Workshop Org

This webinar will cover:

• Read Quality Control 
• Process Reads (Trim/Filter)
• Read-based taxonomy
• Re-check processed reads 
• Set-up assembly run(s)

Learning Goals

• Establish a standard for data quality; why sequences need to be trimmed/filtered after sequencing,
• Interpret read quality and review standards, and
• Understand the reasoning behind testing multiple assembly tools.
  

Materials
Slides
Template Narrative

09:00 Compare assemblies
09:45 Set-up binning tools
10:15 Review samples & data context
11:15 Interpret read taxonomy
Lunch
13:00 Interpret bin optimization
13:45 Assess bin quality
15:00 Filter for medium/high quality bins
16:00 Set up functional annotation

Learning Goals

• Understand and interpret relative quality of assemblies
• Understand the concept of binning and the reasoning behind optimizing multiple tools 
• Compare read-based taxonomy to amplicon data
• Understand bin optimization results
• Define bin quality and cut-offs
• Define metagenome-assembled genomes

Materials
Slides

09:00 Set up metagenome-assembled genome taxonomy
09:30 Interpret functional annotation
10:30 Interpret taxonomy
11:15 Build phylogenetic trees
12:00 Lunch
13:00 Synthesis & data in context
14:00 Review how to set up a scientific presentation; Presenting results; Asking questions
14:30 Work Time: presentation preparation

Learning Goals

• Interpret DRAM (functional annotation) results
• Understand estimation of taxonomic classification of bins
• Overall interpretation of results
• Develop conclusions and insights with context of data
• Develop questions from results

Materials
Slides

09:00 Set-up
10:00 Student Presentations

Learning Goal

• Explain results and findings with contextual information to peers