Celebrating user wins and working with the community!
Jul 10, 2025

Quarterly User Update (April – June 2025)

Welcome to our community update for Q2 2025 – covering announcements and highlights; and celebrating accomplishments from our team, users, and collaborators.

American Society for Microbiology offered a training series on KBase

KBase collaborated with the American Society for Microbiology to provide hands-on, practical training for instructors and early career researchers in microbial data analysis. The series went step-by-step through the process of microbial isolate genome analysis from raw sequencing reads to assembling and annotating genomes in the Narrative. The last session in the series covered how to prepare an ASM Microbiology Resource Announcement data publication using resources from KBase (https://asm.org/webinars/from-bench-to-publication-identification-and-analy).

Microbial Isolate Genomics workflow. Shows steps from data import and utilities to uploading to KBase. Then the workflow from Read Processing, Genome Assembly, Genome Annotation, Microbe Classification, Sequence Analysis to Build a metabolic model. The last steps after analysis in KBase are to export data, create a static Narrative, and publish.

Microbial Isolate Genomics workflow followed during the From Bench to Publication series.

While this first series ended in May, there is an upcoming one beginning on September 2 covering community-wide metagenomic data, with live webinars and hands-on practice. Details here: https://asm.org/webinars/from-sample-to-mag-identifying-and-characterizing

Community Highlights

Group photo of workshop participants at University of Massachusetts Amherst standing outside in front of green trees and a lawn.

Workshop participants at University of Massachusetts Amherst.

In May 20-22, the first regional MICROnet Training Workshop took place at the University of Massachusetts Amherst with 16 participants from 11 institutions across the Northeast (https://www.kbase.us/news/micronet-jun-2025/). These instructors will be part of the first regional MICROnet cohort integrating microbiome research modules into their courses during the 2025-26 academic year. Stay tuned to hear more about the outcomes and program milestones! 

Two sessions at the American Society for Microbiology Microbe meeting in Los Angeles, California highlighted KBase and platform resources. KBase co-hosted a session with ASM Microbiology Resource Announcements to discuss FAIR – Findable, Accessible, Interoperable, Reusable – data principles and leveraging the landscape of persistent identifiers in a Lounge and Learn. KBase user Hannah Schweitzer presented on the Produced Water DNA Database (PW-DNA). This resource is readily available in KBase and open to all users! 

For questions about using the PW-DNA database or comments and feedback, please reach out to us at engage@kbase.us.

KBase Publications

The platform and team behind KBase continues to support users to analyze their data and follow the FAIR (findable, accessible, interoperable, reusable) data principles through to publication. Studies published this quarter included:

  • Environmental contexts with how denitrifying bacterial isolates can disrupt denitrification in contexts such as high nitrate sites (Carr et al. 2025); 
  • How diet has a greater impact in restoring dysbiosis in mice compared to microbiota transplants (Kennedy et al. 2025); and 
  • Making tools for multi-omic network analysis using the RWRtoolkit (random walk with restart toolkit) available on KBase (Kainer et al. 2025).  

All publications using KBase are available at: https://www.kbase.us/research. If we are missing your publication citing KBase in your methods, please let us know at engage@kbase.us.

Summary

To close out our update, here is a summary documenting April through June 2025 user numbers. Keep doing great science in KBase. The KBase team is here to support you!

Statistics for KBase from January to March 2025. New User Accounts grew by 1,981 for a total of 47,065. User Narratives grew by 4,275 for a total of 114,000. User data grew by 57.5 terabytes for a total of 1,286 terabytes. Public data grew by 147 gigabytes to 23.2 terabytes total. There were 37 new publications citing KBase for 793 publications since 2014. Top App categories: annotation, assembly, and communities. KBase.us had 56,000 site visits with 16,000 new visitors and 13,700 active users. Help Board tickets included 3 Feature Requests, 7 Closed Questions, 10 Open Questions, 10 Open Bugs and 12 Closed Bugs. The KBase YouTube Channel had 3,300 views, 34 new subscribers for 1,417 total subscribers. LinkedIn had 2,339 Impressions, 33 Page Views, and 499 Followers.

KBase by the numbers from the April through June 2025 Quarter Report.

Ellen Dow
Ellen Dow
Lawrence Berkeley National Laboratory

Ellen G. Dow, Ph.D. leads the KBase Educators Program as part of the User Engagement team. Inspired by her involvement in science outreach throughout graduate school, she left the bench to gain experience in informal education and cultivate community engagement from public to science sectors. A molecular biologist by training, Ellen applies her research experience to support emerging scientists and co-developing community resources.