Bacterial-Fungal Interactions SFA
Integrating fungal computational sequence data analysis capabilities
The Bacterial-Fungal Interactions Science Focus Area (SFA) driven by Patrick Chain at Los Alamos National Laboratory (LANL) aims to address some of the knowledge gaps around bacterial-fungal interactions within the soil. The researchers are using a multi-omics approach to gain a predictive understanding of the bacterial-fungal interactions. This work will shed light on how microbial interactions in soil affect ecosystem services such as plant productivity, nutrient cycling, and carbon flux.
Collaborative Development Objectives
Data analysis modules:
- Bulk retrieval of fungal sequencing data, from raw data to draft contigs to finished genome
- Determination of unique genomic signatures at each taxonomic rank for fungal genomes
- Specific detection of microbial species based on unique fungal signatures
Tools:
- GOTTCHA2 (Genomic Origin Through Taxonomic Challenge): Uses a novel taxonomic profiling method for metagenomes that is both gene-independent and signature-based. It significantly reduces false discovery rates (FDR). (Released)
- Centrifuge Taxonomic Classifier: Enables rapid, accurate, and sensitive labeling of metagenomic reads and quantification of species. (Beta-only)
- Kraken2 Taxonomic Sequence Classifier: Examines the k-mers within a query sequence and maps k-mers to the lowest common ancestor (LCA) of all genomes in the reference database known to contain a given k-mer. (Beta-only)
Additional Information
Meet the Members
SFA Manager/PI: Patrick Chain1
KBase Contact: Paramvir Dehal5,6
Lead Developers: Mark Flynn1, Po-E Li1, and Chien-Chi Lo1
Project Team: Michal Babinski1, David Bruce1, Jean Challcombe1, James Werner1, Armand Dichosa1, Migun Shakya1, Geoffrey House1, Jamey Young2, Pilar Junier3, Debora Rodrigues4
Affiliations: 1Los Alamos National Laboratory, 2Vanderbilt University, 3University of Neuchâtel, Switzerland, 4University of Houston, 5Lawrence Berkeley National Laboratory, 6KBase
Resources
- GitHub Repository
- Empowering the Development of Genomics Expertise (EDGE) Website
- Taxonomy Tools (21 April 2021) – KBase tutorial webinar
- KBase Taxonomy Tools Documentation