Microbes Persist SFA

Foundation for Understanding and Prediction of Microbial and Viral Ecogenomics in Soil

The Microbes Persist Soil Microbiome Science Focus Area (SFA), led by Dr. Jennifer Pett-Ridge at Lawrence Livermore National Laboratory (LLNL), aims to understand the critical role that microbes play in the stabilization and persistence of soil carbon. Microbes Persist does so by studying microbial biochemistry, functional potential, and physiology of the soil microbiome. This SFA is producing hundreds of stable isotope probing genome-resolved metagenomes and will be implementing new tools in KBase to tackle the bioinformatic challenges associated with the corresponding data analysis. In addition, collaborations with the Banfield group (University of California, Berkeley) addresses microbial metagenome curation efforts, and the Sullivan group (The Ohio State University) has contributed iVirus tools to KBase.

Collaborative Development Projects

Starting Fall 2021 – Quantitative Stable Isotope Probing (qSIP) workflow

Starting Fall 2021 – Integration of microTrait genomes-to-train workflow

Metagenomic Functionality:

  • FixAME enables curation of assembly quality in metagenome bins (in Development)

Viral Tools:

    • Two tools from iVirus, a tool designed to explore viral ecology and bring bioinformatic analyses to the study of viral genomes
    • VirMatcher predicts virus-host relationships using variety of bioinformatic methods: host CRISPR-spacers, integrated prophage, host tRNA genes, and k-mer signatures calculated by WIsH. (Released in KBase)

  • PhATE, a phage functional annotation pipeline developed at LLNL (in Development)

Additional Information

Meet the Members

SFA Principal Investigator: Jennifer Pett-Ridge1
SFA Contact: Jennifer Pett-Ridge1
KBase Contacts: Paramvir Dehal2,3 and Dylan Chivian2,3
Lead Developers: Ben Bolduc4, Jeff Kimbrel1, Ulas Karaoz2, Ben Koch5, Gianna Marschmann1, Rohan Sachdeva6
Project Team: Jill Banfield2,6, Eoin Brodie2, Bruce Hungate5,  Matthew B. Sullivan4
Affiliations: 1Lawrence Livermore National Lab, 2Lawrence Berkeley National Lab, 3KBase, 4The Ohio State University, 5Northern Arizona University, 6University of California, Berkeley,

Resources